Journal: Neuron
Article Title: Rbfox splicing factors promote neuronal maturation and axon initial segment assembly
doi: 10.1016/j.neuron.2018.01.020
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: NA NA Recombinant DNA pCAGGS-Cas9-mCherry This paper pgRNA empty Addgene #41824 pgRNA Rbfox1 KO This paper pgRNA Rbfox2 KO This paper pgRNA Rbfox3 KO This paper pgRNA1 AnkG del This paper pgRNA2 AnkG del This paper pgRNA1 AnkG ins This paper pgRNA2 AnkG ins This paper pCAGGS-3xFLAG-Rbfox1 This paper pCAGGS-3xFLAG-Rbfox2 This paper pCAGGS-3xFLAG-Rbfox3 This paper pCAGGS-3xFLAG-bIV spectrin (repeats 13–15) This paper pCAGGS-3xFLAG-bII spectrin (repeats 13–15) This paper pCAGGS-AnkG ZU5in-3xHA This paper pCAGGS-AnkG ZU5ex-3xHA This paper pCAGGS-AnkB ZU5ex-3xHA This paper Software and Algorithms AIS quantification algorithm (Matlab) ( Grubb and Burrone, 2010 ) (ais_z3.m) http://grubblab.org/resources/ Fiji ( Schindelin et al., 2012 ) https://fiji.sc/ RNA-seq analysis by Quantas ( Yan et al., 2015 ) http://zhanglab.c2b2.columbia.edu/index.php/Quantas CLIP data analysis by CTK ( Shah et al., 2017 ) http://zhanglab.c2b2.columbia.edu/index.php/CTK Open in a separate window KEY RESOURCES TABLE EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell Culture Mouse embryonic stem cells (ESCs) were cultured in EmbryoMax DMEM (EMD Millipore) supplemented with 15% embryonic stem cell screened fetal bovine serum (HyClone), 2mM L-glutamine (Life Technologies), 1x non-essential amino acids EmbryoMax MEM (EMD Millipore), 1x EmbryoMax nucleosides (EMD Millipore), 0.1mM β-mercapthoethanol (Sigma-Aldrich), 100U/ml penicillin-streptomycin (Life Technologies), 1000U/ml ESGRO Leukemia inhibitory factor (EMD Millipore), 2.5μM GSK-3 inhibitor XVI (EMD Millipore) and 50nM FGF receptor antagonist PD173074 (Tocris).
Techniques: Produced, Recombinant, Software
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